Thèmes de recherche :

Mes recherches on porté sur les polysaccharides et les microorganismes.
Après avoir rejoint le LECA, mes recherches concernent la microbiologie environnementale. Le but de ces recherches est d’évaluer l’impacte des activités humaines sur les micro-organismes des systèmes naturelles, surtout en ce qui concerne le cycle de C.

CV :

1983-1989 :Thèse à l’Instituto de Investigaciones Bioquìmicas, Fundación Campomar dirigé par le Prof. L. F. Leloir (Prix Nobel 1991), Buenos Aires, Argentine.
19891993 : Stage Postdoctoral au Laboratorium voor Genetika de l’Université de Gand dirigé par le Prof. M. van Montagu, Belgique.
1993-2000 : Responsable du groupe «Glycobiologie Moléculaire» du ", Centre de Recherches sur les Macromolécules Végétales (CERMAV).
2000-2004 : Laboratoire de Plasticité et Expression des Génomes Microbiens, FRE 2029 UJF-CNRS, puis Laboratoire "Adaptation et Pathogénie des Micro-Organismes » UMR5163.
Depuis 2004 : Laboratoire d’Ecologie Alpine, Equipe Perturbations Environnementales et Xénobiotiques, Microbiologie Environnementale et Biodiversité.

Projets :

Dynamique spatiotemporel de la microflore des écosystèmes alpins (TDE et GPB).
Programme EMMAUS.
Collaborations : J. Martins (LTHE), J.P. Bedell et C. Delolme (LES, ENTPE).

Publications :

Baptist F, Zinger L., Clement J.C., Gallet C., Guillemin R., Martins J.M.F., Sage L. 1, Shahnavaz B., Choler Ph., Geremia R. A. (2007). Tannin impacts on microbial diversity and the functioning of alpine soils. Environmental Microbiology, in press.

Zinger L., Gury J., Alibeu A., Rioux D., Gielly L., Sage L., Pompanon F. Geremia R.A. (2007). CE-SSCP and CE-FLA, simple and high throughput alternatives for fungal diversity studies. Journal of Microbiological Methods, in press.

Zinger L., Gury J., Giraud F., Krivobok S., Gielly L., Taberlet P., Geremia R.A. (2007). Improvements of PCR and CE-SSCP methods in microbial ecology: towards a high-throughput method for microbial diversity studies in soil. Microb. Ecol. 54:203-216.

Chalmin E., d’Orlié F., Zinger L., Charlet L., Geremia R.A., Orial G., Menu M., Baffier D., Reiche I. (2007)Biotic vs. abiotic calcite formation on prehistorical cave paintings: the Arcy-sur-Cure “Grande Grotte” (Yonne, France) case. In: Natural & anthropogenic hazards in karst areas:Recognition, Analysis, Mitigation, Ed. By M. Parissi and J. Gunn (ISBN 978-1-86239-224-3) Geological Society Publishing House, London.

Saksouk N, Pelosi L, Colin-Morel P, Boumedienne M, Abdian PL, The capsular polysaccharide biosynthesis of Streptococcus pneumoniae serotype 8: functional identification of the glycosyltransferase WciS (Cap8H). Biochem J. 389:63-72.

Bettler E., Imberty A., Priem B., Chazalet V., Heyraud A. Joziasse D. H., Geremia R.A. (2003). Production of recombinant oligosaccharidic xenoantigen. (2003). Biochem Biophys Res Commun 302, 620-624.

CidE., Geremia R.A. , Guinovart J. J., Ferrer J. C.. (2002). Glycogen synthase: towards a minimum catalytic unit? FEBS Letters, 528:5-11. Videira P., Fialho A., Geremia R.A.*, Breton C., Sa-Correia I. (2001). Biochemical characterisation of the ß (1-4) glucuronosyltransferase GelK in the gellan gum producing Sphingomonas paucimobilis ATCC 31461. Biochem. J. 358: 457-464.

E. Cid, R. R. Gomis, Geremia R.A., J. J. Guinovart, J. C. Ferrer. (2000). Identification of Two Glutamic Acid Residues at the Active Site of Glycogen Synthase. J. Biol. Chem 275: 33614-33621.

Abdian P., A. C. Lellouch, C. Gautier, L. Ielpi, Geremia R.A. (2000). Identification of essential amino acids in the bacterial a-mannosyltransferase AceA. J. Biol. Chem. 275:40568-40575.

Schneider C., A. C. Lellouch, Geremia R.A. (2000). Identification of essential amino acids in the bacterial ß-glucosyltransferase ExoM. J. Biol. Chem. 275, 31407–31413.

Lellouch A.C., Geremia R.A. (1999). Expression and study of recombinant ExoM, a ß 1-4 glucosyl transferase involved in succinoglycan biosynthesis in Sinorhizobium meliloti. J. Bacteriol. 181:1141-1148.

F. Gressent, Drouillard S., Mantegazza N., Samain E., Geremia R.A., Canut H., Niebel A., Driguez H., Ranjeva R., Cullimore J., Bono J.J. (1999). Ligand specificity of a high-affinity binding site for Lipo-chitooligosaccharidic Nod factors in Medicago cell suspension cultures. Proc. Natl. Acad. Sciences USA, 96:4704-4709.

Samain E., Drouillard S., Heyraud A., Driguez H., Geremia R.A. (1997).Gram scale synthesis of recombinant chitooligosaccharides in Escherichia coli. Carbohydr. Res., 302:35-42.

Geremia R.A., Harduin-Lepers A., and P. Delannoy. (1997). Identification of Two Novel Conserved Amino Acid Residues in Eukaryotic Sialyltransferases; Implications for their Mechanism of Action. Glycobiology, 7: v-xi.

Geremia R.A., E. A. Petroni, L. Ielpi, and B. Henrissat. (1996). Toward a classification of glycosyl transferases based on amino acid sequence similarities. Prokaryotic a-mannosyl-transferases. Biochem. J. 318:133-138.

Saxena, I.M., Brown, R.M., Fevre, M., Geremia R.A., Henrissat, B. (1995). Multidomain architecture of ß-glycosyltransferases: implications for mechanism of action. J. Bact. , 177:1419-1424.

Geremia R.A., Mergaert P., Geelen D., Van Montagu M. & Holsters M. (1994). The NodC protein of Azorhizobium caulinodans is a N-acetylglucosaminyl transferase. Proc. Natl. Acad. Sciences USA ,91, 2669-2673.

Geremia R.A., Goldman G.H., Jacobs D., Ardiles W., Vila S.B., Van Montagu M. & Herrera-Estrella A. (1993). Molecular characterization of the proteinase-encoding gene, prb1, related to mycoparasitism by Trichoderma harzianum. Mol. Microbiol. 8, 603-613.

Geelen D., Mergaert P., Geremia R.A., Goormachtig, S., Van Montagu M. & Holsters M. (1993). Identification of nodSUIJ genes in Nod locus 1 of Azorhizobium caulinodans: evidence that nodS encodes a methyltransferase involved in Nod factor modification. Mol. Microbiol. 9, 145-154.

Geremia R.A., Cavaignac S., Zorreguieta A., Toro N., Olivares J. & Ugalde R.A. (1987). A Sinorhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharide but fails to form ß (1?2) glucan. J. Bacteriol. 169, 880-884.

Zorreguieta A., Geremia R.A., Cavaignac S., Cangelosi G.A., Nester E.W. & Ugalde R.A. (1988). Identification of the product of an Agrobacterium tumefaciens chromosomal virulence gene. Mol. Plant Microbe Interactions 1, 121-127.